Computer modeling of nitroxide spin labels on proteins
نویسندگان
چکیده
منابع مشابه
Hydrogen bonding of nitroxide spin labels in membrane proteins.
On the basis of experiments at 275 GHz, we reconsider the dependence of the continuous-wave EPR spectra of nitroxide spin-labeled protein sites in sensory- and bacteriorhodopsin on the micro-environment. The high magnetic field provides the resolution necessary to disentangle the effects of hydrogen bonding and polarity. In the gxx region of the 275 GHz EPR spectrum, bands are resolved that der...
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Chloroplasts isolated from oats eliminated the electron spin resonance (ESR) signals from spin labels in white light and partially restored them in far-red light. Only the white light-mediated reaction was blocked by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). In contrast, oat (Avena sativa L. cv. Garry and Park) leaf mesophyll protoplasts oxidized the spin labels in both white and far-red ...
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1. 12-Nitroxide stearate binds to bovine serum albumin at about four independent and equivalent binding sites with an association constant of about 10(6) M-1. The binding at these high affinity binding sites is significantly reduced by addition of unlabeled stearate. These data suggest that nitroxide stearates probe the high affinity binding sites for long-chain fatty acids. 2. Qualitative anal...
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Long-range distance measurements based on paramagnetic relaxation enhancement (PRE) in NMR, quantification of surface water dynamics near biomacromolecules by Overhauser dynamic nuclear polarization (DNP) and sensitivity enhancement by solid-state DNP all depend on introducing paramagnetic species into an otherwise diamagnetic NMR sample. The species can be introduced by site-directed spin labe...
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The rotational motion of an iSN nitroxide in diisobutylphthalate solutions at varied temperatures has been studied for correlation times from 1Om8 to > 10m3 sec. Data from both conventional (first-harmonic, in-phase) and saturation-transfer (second-harmonic, out-of-phase) EPR are reported. For many spectral parameters, including linewidth, position of outer extrema, and ratios of spectral ampli...
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ژورنال
عنوان ژورنال: Biopolymers
سال: 2011
ISSN: 0006-3525
DOI: 10.1002/bip.21699